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AutoFlow NU-4950 Oxygen and Humidity Control Water Jacket CO2 Incubator

The AutoFlow 4950 Oxygen and Humidity Control Water Jacket Laboratory CO2 Incubator creates an in-vitro growth environment for research and clinical applications involving stem cell cultures, cancer cells, oncology, hypoxia, toxicity, microbiology and more.

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Oxygen Control System

The O2 control system uses a fuel cell oxygen sensor to constantly monitor, control, and display oxygen levels from 21% down to 2% with accurate chamber readings and rapid recovery times.

Humidification System

The RH control system uses a solid state capacitance humidity sensor to monitor, control, and display humidity by injecting heated water vapor to your setpoint from a reservoir outside the chamber giving the operator control over the amount of humidity from below ambient to 95%.

Closed Loop HEPA Filtration

Limits the need for moving parts minimizing vibration. An air pump injects filtered air into the work chamber. The air recirculates through sensor checkpoints before it is re-injected into the work chamber passing through a 99.99% HEPA filter. This system slows air movement to one chamber air change every 30 minutes. The decelerated air movement ensures ISO class 5 air conditions to minimize cell desiccation by airflow.

NICE Electronics

NuAire Incubator Control Electronics (NICE) is a state-of-the-art microcomputer based control system specifically designed to service the precise control requirements of the chambers environment, providing optimum programmable conditions for culture growth.

Constant Contamination Control

A unique HEPA filtration system filters fresh air into the pump, CO2 gas from tanks or house systems, IR CO2 sensor sample air returning to the change, air between air pump and inner chamber, while continuously filtering the internal chamber to create ISO Class 5 air quality.

Cleanroom within a Cleanroom

Interior chamber is constantly maintained at positive pressure similar to an ISO Class 5 Cleanroom.

Sensitivity and Accuracy of Gas Control

Infrared (IR) CO2 sensor provides stable outputs requiring less calibrations.

Precision of Electronic Microprocessor Controls

A programmable microcontroller samples input sensors, sets control outputs, and makes necessary corrections to the chamber environments resulting in quick and responsive recovery times.

Reliability and Protection of Automated and Redundant Back-up Systems

Dual temperature sensor probes, solid-state analyzers, HEPA filtration systems, and status alarms create a dependable, long lasting scientific instrument.

Critical Cleaning Components

Coved corners prevent contaminants from becoming trapped in the chamber. Removable shelves, brackets, and gasket make cleaning simple by cleaning around them or placing them in the autoclave.

Homogeneous Temperature

All 6 sides of the chambers walls feature heating elements surrounded by R5 insulation to provide optimum temperature uniformity in the work chamber.

  • 100% Stainless Steel Interior
  • 4 Removable Stainless Steel Shelves
  • 20 Gallon (75.7 L) Water Jacket
  • Remote Alarm Output Contacts
  • CO2 Sample Port
  • Adjustable Leg Levelers
  • Access Port

Optional Features

  • Antibacterial Copper Enriched Shelves
  • Internal Tank Switch
  • RS-232 Communications Interface
  • Multi-Signal Analog Outputs
  • Additional Shelves
  • Cooling Coil
  • 4 Segmented Inner Lexan Doors
  • Right Door Swing
  • Water Pan
Model NU-4750
Chamber Volume 6.65 ft.3 / 188 L
Exterior Dimensions (W x D x H)
in. [mm]
31 x 25 ½ x 34 (787 x 648 x 864)
Chamber Dimensions (W x D x H)
in. [mm]
21 ½ x 21 x 25 ½ (546 x 533 x 648)
Weight 222 lbs. / 101 kg.
Temperature Control System  
Range 18°C to 55°C
Sensitivity ± 0.125°C
Uniformity ± 0.2°C at 37°C
Accuracy ± 0.0125°C
Display Resolution 0.1°C
CO2 Control System  
Range 0.1 to 20%
Accuracy ± 0.1%
Recovery 5.0% ± 2.0% in 3 minutes 30 seconds
Uniformity ± 0.1%
Display Resolution ± 0.1%
Humidity Control System  
Range 5 % above ambient to 95 %
Accuracy ± 3.0 %
Recovery 95 % ± 3.0 % within 10 minutes
Oxygen Control System  
Range 2 to 21 %
Accuracy ± 1.0 %
Recovery 5.0 % ± 2.0 % within 10 minutes
Warranty 2 Year Parts and Labor (US & CA)
  3 Year Parts (Intl)
Accolades UL, UL-C, CE, Energy Saver, RoHS, Constant Contamination Control, Critical Cleaning Components
Please note specifications are subject to change. Please contact NuAire to verify before purchase.