NuAire recommends the use of this CO2 Incubator humidity water pan for when copper components are desired as a part of the chamber contamination control. Place the pan on the bottom of the incubator chamber. The pan should be surface disinfected before usage with 70% alcohol or similar disinfectant. Fill with single deionized or distilled water (No purer than 1 mega ohm). No other additives are required for effective antimicrobial properties.