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In-VitroCell ES NU-5741 5.65 cu. ft. (160L) Hypoxic Direct Heat CO2 Incubator with Dual Sterilization Cycles, Humidity, and O2 Control

Model NU-5741 is a Direct Heat CO2 Incubator featuring precise Humidity control through the use of a water reservoir and sensor as well as Oxygen control for hypoxia research by injecting Nitrogen gas to meet set point to suppress Oxygen levels.

Part #: NU-5741


The In-VitroCell™ ES NU-5741 5.65 Ft3 (160 L) Hypoxic Direct Heat CO2 Incubator is designed to provide labs with a controlled in-vitro environment for age related stuides or stem cell research. The NU-5741 provide optimum growth conditions by precisely controlling humidity, temperature, oxygen and CO2 by monitoring and controlling the growth environment.

Standout Technology

NuTouch Electronic Control System

The NuTouch Electronic Control System of the In-VitroCell™ ES NU-5741 5.65 Ft3 (160 L) Direct Heat CO2 Incubator makes controlling it easy. The NuTouch control system is a color, touchscreen system, so you can easily control the system's parameters with just the tap of a finger.

Hypoxic Conditions

The chamber of the incubator can offer hypoxic conditions, using a sensor for O2 control and injecting N2 gas to meet a setpoint in a range of 0.5 percent to 21 percent, accurate with ±0.25 percent.

Precise Contamination Control

Dual Sterilization Cycles

The NU-5741 incubator is engineered with dual sterilization cycles to protect both products in the chamber and personnel in the lab. The dual cycles are at 145°C dry and 95°C humidified.

Designed for Protection

The incubator was designed with a coved corner and crevice-free interior, which makes it both easy to clean and limits potential hiding places for contamination.

Closed-Loop HEPA Filtration

The closed-loop HEPA filtration system in the NU-5741 model incubator was also designed to minimize the risk of contamination. The system is similar to that of an ISO class 5 clean room, maintained at a positive pressure, with gas and air continuously passing through 99.99 percent efficient HEPA filters.

No Vibration

The incubator has no vibration since there are fewer moving parts. The reduced vibration helps protect cell lines.

Unique Performance Features

Humidity Control

Humidity control in the NU-5741 is managed through a three-liter reservoir that injects water vapor to meet a set point up to 95 percent. This system is used in the incubator in place of a user water pan. The humidity control system is accurate within +5 percent / -3 percent.

Faster Recovery Times

The NU-5741 incubator features faster recovery times after door openings, which helps maintain temperature, CO2 gas and O2 levels.

Accurate CO2 Levels

There is a dual wave infrared sensor in the incubator that helps maintain proper CO2 concentrations in the growth chamber, accurate within ±0.1 percent. The sensor is insensitive to other components, such as water vapor.

Uniform Temperature

The uniform temperature in the incubator (±0.3C) is monitored using dual temperature probes.

Standard Features

  • NuTouch Electronic Control System
  • Closed Loop HEPA Filtration System
  • Dual Sterilizaiton Cycles (145°C Dry, 95°C Humidified)
  • 16-Gauge Type 304 Polished Stainless Steel Growth Chamber
  • 16-Gauge Type 304 Polished Stainless Steel Shelving (Qty 4)
  • 16-Gauge Type 304 Polished Stainless Steel Brackets (Qty 4)
  • 16-Gauge Type 304 Polished Stainless Steel Shelf Guides (Qty 8)
  • Infrared (IR) Sensor
  • Humidity (RH) Resviour and Control System
  • Stainless Steel Water Pan for installation and calibraiton only
  • O2 Monitor and Control System
  • CO2 Sample Port
  • R5 Insulation System
  • Independent Door Heater
  • Right Hinge Swing Field Reversible Exterior Door
  • Right Hinge Swing Field Reversible Inner Glass Door
  • Removable/Autoclavable Inner Door Gasket
  • Heavy Duty Leg Levelers
  • USB Port
  • RJ-11 Communication Port for Remote Alarm Connection
  • RJ-45 Jack - RS-485 2-way Communication
    • Output: Control system levels and alarms/events
    • Input: Commands from a Computer to view and update operating parameters
  • RJ-45 Jack - 4-20 mA Analog Output for monitoring all active control system levels
  • Utility Access Port
  • Electrical power cord
  • Field Stackable with Appropriate NuAire Incubator Models

Parts & Accessories
  • Copper Shelves
  • Copper Chamber
  • Left Hinged Exterior Door
  • a
  • Left Hinged Interior Door
  • Gas Tight Sectioned Inner Door

Electrical Requirements


NU-5741: 115V, 50/60Hz

NU-5741E: 230V, 50/60Hz

Quality Assurance UL, UL-C, CE


Exterior Dimensions (W x D x H)

25.5 x 27.5 x 36.188 in

648 x 699 x 894 mm

Foot Print (W x D)

22.625 x 20.825in

575 x 530 mm

Interior Dimensions (W x D x H)

20.25 x 20.678 x 24 in

514 x 525 x 610 mm

Shelf Dimensions (W x D)

18 x 18.75 in

457 x 476 mm

Shelves Supplied (Standard) Quantity 4
Maximum Shelf Capacity


Maximum Weight Capacity

25 lbs (11.34 kg) per shelf

125 lbs (56.7 kg) per incubator

Water Pan Dimensions (W x D x H)

10 x 1.5 x 12 in

254 x 38 x 305 mm

Water Pan Capacity

2 Liters (Maximum)

1.5 Liters (Recommened Fill)


5.65 ft3

160 L

Net Weight

225 lbs

103 kg

Temperature Control

Control Range:

5°C to 55°C (37°C default) (5°C above ambient to 30°C maximum ambient)

Set-Point Range: 5°C to 55°C (37.0 Default)
Uniformity: ±0.3°C @ 37°C
Accuracy: ± 0.1°C
Recovery: 0.12°C/min. on Average
Display Resolution: 0.1°C
Door and Perimeter Heater Control Logic: Proportional base duty cycle based on Temperature set point and -20 to +20% manually adjustable to adapt to ambient conditions.
Temperature Sensor Type: Precision Integrated Circuit

CO2 Control Standard


0.1 to 20% (default 5%). (0.0 set point idles system)

Accuracy: ±0.1%
CO2 Recovery: Up to 5% ±0.2% / -0.5 in 5 minutes Average
CO2 Display Resolution: 0.1%
CO2 Control Logic: Fixed Algorithm/Manual, Environmental Adaptable
CO2 Sensor Type: Infrared single source dual wave length

Relative Humidity Display and Control

Control Range:

Ambient dependent to 90%

RH Accuracy: ±3%
RH Recovery: 90% +5%/-3% within 25 minutes on average
Display Resolution: 0.1%
RH Control Logic: Fixed algorithm/manual environmental adaptable
RH Sensor Type: Precision capacitance bulk-polymer

Oxygen Display and Control

Control Range:

0 to 25%

O2 Set-Point Range 0.5 to 21%
Default Set Point 21%
O2 Accuracy: ±0.25%
O2 Recovery: 5% ±2%: 15 minutes on average

Download Resources
In-VitroCell CO2 Incubators


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