In-VitroCell ES NU-5820 Direct Heat 7 ft3 (200L) CO2 Incubator with Humidity Control

The In-VitroCell ES NU-5820 Microbiological CO2 Incubator features relative humidity (RH) control for cell lines prone to desiccation by utilizing a sensor that measures humidity in the chamber and injects 99.99% HEPA filtered vapor into the growth chamber to maintain setpoint.

Part #: NU-5820


Promote cell growth while minimizing potential contamination with this In-VitroCell ES humidity control CO2 incubator. Ideal for cell lines prone to desiccation, this incubator accurately measures and controls relative humidity.

Constant Contamination Control

Closed-Loop HEPA Filtration

Ensure constant contamination control with a multistep process in a closed system. An air pump, HEPA filtration, and IR and RH sensor bay make up the closed loop, all controlled by the NuTouch ECS. The closed-loop system lowers airflow, so air is changed only once every 20 minutes, minimizing desiccation.

Keep Contaminated Lab Air Out

Growth chamber air is maintained at a positive pressure, similar to an ISO 5 cleanroom, preventing contaminated lab air from being drawn into the chamber when the door is open. Any air or gas entering the chamber must first pass through the 99.99 percent HEPA filters.

Dual Sterilization Cycles

Keep the incubator chamber sterile with dual sterilization cycles. Use 145 degrees C high-heat sterilization or 95 degrees C humidified decontamination to eradicate contamination. Sterilization cycles can be set to run on a timer using the NuTouch ECS.


Contamination-Preventing Design

Prevent contamination from the start with this crevice-free chamber constructed of high-grade 16-gauge stainless steel. The seamless coved interior corners are easy to clean, and the inert surface does not promote biological growth. A single-piece gasket creates a liquid-tight seal from incubator chamber to inner door to eliminate condensation build-up and potential contamination. You can also remove the inner chamber shelving and gasket for autoclaving.

Minimal Vibration Promotes Cell Growth

The maintenance-free air pump, combined with minimal moving parts, eliminates vibration that could disturb cell growth.


Sensitivity and Accuracy of CO2 Control

Accurately control the level of CO2 in the growth chamber with the single-source dual-wave infrared (IR) sensor. This nondispersive sensor is insensitive to components other than CO2 and creates a stable output that minimizes drift and requires less calibration.

NuTouch Electronic Control System

Program the optimum conditions for culture growth with the NuTouch electronic control system (ECS). This user-friendly touch screen system lets lab workers quickly input and monitor temperature and CO2 following the step-by-step onscreen instructions. The screen displays system status throughout the incubation process, including sounding an alarm if there's a system failure.


Relative Humidity Control

Maintain relative humidity up to 95 percent in the incubator. A reservoir under the chamber holds water, which is vaporized and injected into the chamber when the continuously sampling RH sensor measures a decrease in humidity.

Temperature Uniformity

Ensure the internal temperature of the incubator never rises or falls more than 0.35 degrees C (at 37 degrees C). Five heating elements surround the chamber, and the door is heated separately to minimize condensation. Dual temperature sensor probes convey information to the NuTouch ECS, which makes adjustments as necessary.

  • Copper Shelves
  • Copper Chamber
  • Left Hinged Exterior Door
  • Left Hinged Interior Door
  • Gas Tight Sectioned Inner Door


Exterior Dimensions (W x D x H)

26.75 x 27.12 x 41.5 in.

679.5 x 689 x 1054 mm

Foot Print (W x D)

23.75 x 20.75 in.

603.2 x 527.1 mm

Interior Dimensions (W x D x H)

21.5 x 21 x 26.5 in.

546.1 x 533.4 x 673.1 mm


7.06 ft3

200 L

Net Weight

235 lbs

106 kg

Temperature Control

Control Range:

5° above ambient (to a 30° max ambient) to 55°

Set-Point Range: 5°C to 55°C (37.0 Default)
Uniformity: ±0.35°C @ 37°C
Accuracy: ± 0.1°C
Recovery: 0.12°C/min. on Average
Display Resolution: 0.1°C
Door and Perimeter Heater Control Logic: Base Duty Cycle set to chamber temperature Set-Point Proportional 0-100% [manually adjustable]
Temperature Sensor Type: Precision Integrated Circuit

CO2 Control Standard


0.1 to 20% 5% Default Set Point

Accuracy: ±0.1%
CO2 Recovery: Up to 5% ±0.2% / -0.5 in 5 minutes Average
CO2 Display Resolution: 0.1%
CO2 Control Logic: Fixed Algorithm/Manual, Environmental Adaptable
CO2 Sensor Type: Infrared (NDIR)

Relative Humidity Display and Control

Control Range:

Ambient dependent to 90%

RH Accuracy: ±3%
RH Recovery: 90% +5%/-3% within 25 minutes on average
Display Resolution: 0.1%
RH Control Logic: Fixed algorithm/manual environmental adaptable
RH Sensor Type: Precision capacitance bulk-polymer

Download Resources
In-VitroCell CO2 Incubators


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