Direct Heat CO2 Incubator

200-Liter Capacity | Direct Heat | Oxygen Suppression | Decontamination Cycles

The In-VitroCell NU-5831 is a Direct Heat Hypoxic CO2 Incubator that suppresses oxygen levels in the growth chamber. In addition, model NU-5831 features a Zirconia Ceramic O2 that monitors Oxygen levels within the growth chamber. When oxygen levels rise above the setpoint, the suppression system engages by injecting Nitrogen (N2) gas to meet the setpoint. The NU-5831 features a 200-Liter capacity chamber and is available with segmented inner doors to help maintain the chamber environment upon door openings. In-VitroCell NU-5831 is the perfect solution for applications involving age-related studies, stem cell research, and more.

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A Product to Meet Your Need

Model NU-5831 is available in two electrical configurations.






Quick Specs

Air Cleanliness (ISO 14644) ISO-Class-5
CO2 Accuracy ± 0.1%
CO2 Recovery Up to 5% ± 0.2% / -0.5 in 5 minutes Average
Control System NuTouch
Temperature Accuracy °C ± 0.1%
Temperature Control Direct Heat
Temperature Recovery 0.12°C/min. on Average
Temperature Uniformity °C ± 0.35°C @ 37°C
  • Features
  • Specs
  • Options & Accessories
  • Resources
  • Citations
CO2 Incubator Oxygen Control for Hypoxic Studies

Hypoxic Conditions

Oxygen Control

For more sensitive cells lines prone to stress and DNA damage, CO2 Incubator models featuring Oxygen control utilize an O2 sensor to monitor oxygen in the growth chamber. Nitrogen (N2) gas is injected to suppress oxygen to set point. An extensive O2 range applies to various research applications, including the brain, eyes, liver, heart, kidneys, and more.


Constant Contamination Control

Advanced features mitigate risks due to contamination. Using a slight positive pressure within the chamber eliminates contaminated laboratory air entering the growth chamber. Air and gas pass through 99.99% @ 0.3 micron efficient HEPA filters before entering the chamber resulting in conditions similar to an ISO Class 5 cleanroom. Smooth rounded corners eliminate potential hiding places for contaminates. An overnight procedure running a decontamination cycle at 145° Celsius dry or 95° Celsius humidified is the perfect solution for the fight against contamination. Contamination control is just one more reason NuAire is the safer choice for your laboratory.


Direct Heat Chamber

The direct heat incubator uses heating elements placed around the chamber, in the door perimeter, and in the door itself to provide balanced temperature within a steel, insulated chamber environment. Door and perimeter heaters can be adjusted independently to ensure uniformity even when the incubator is installed in more demanding ambient temperatures.


Closed Loop HEPA Filtration

Continual HEPA filtration of chamber air complements periodic high heat decontamination to provide Constant Contamination Control. InVitrocell’s design constantly takes a sample of air from the chamber, routes it through a HEPA filter in a compartment outside that inner chamber, and ultimately returns it to create an ISO Class 5 level of clean air in the interior as well as a slight positive pressure. Inside the InVitrocell, your cell cultures are protected by a multi-tiered defense against contamination.

CO2 Incubator Dual Wave Infrared (IR) Sensor

Optimal CO2 Levels

Infrared (IR) Sensor

A single source dual wave infrared (IR) sensor controls CO2 levels within the growth chamber to maintain an optimal PH. The wavelengths used are absorbed only by CO2, making the measurements insensitive to other components such as water vapor. Advanced design provides a stable output minimizing drift, and requires less frequent calibrations.

The NuAire© Advantage

NuAire® utilizes an independent network of sales and service professionals offering our customers a vast array of knowledge and relationships. Our inside sales and service teams are the pride of the industry; some of which have been with NuAire® since the beginning in 1971. We work hard and diligently to make you happy. That is our mission, and that is why we come to work every day. Purchase a NuAire® and become part of our family.


Backed by the Industry's Best

  • 3 Years Parts & Labor, IR Sensor 5 Years Parts Only (United States and Canada)
  • Consult Local Distributor (Global)

Standard Features

  • Cold Rolled Steel with Powder Coated Finish Exterior
  • Polished, 16 Gauge Type 304L Stainless-Steel Chamber
  • Set of 4 Inner Shelves
  • Inner Door
  • Separately Adjustable Perimeter & Door Heaters
  • Infrared CO2 Sensor
  • Dual Temperature Sensors
  • Air Pump External to Chamber
  • HEPA Filter External to Chamber
  • Multilingual Touch Screen
  • USB Port for Data Download
  • Zirconia O2 Sensor
Est. Shipping Width Inches (mm) 31 (787)
Est. Shipping Height Inches (mm) 51 (1219)
Est. Shipping Depth Inches (mm) 31 (787)
Access Port Location Right Side
Air Cleanliness (ISO 14644) ISO-Class-5
Chamber Air Circulation Method Air Pump
Chamber Air Filter 99.97% Efficiency @ ≥ 0.3 Micron Capsule Filter Located in Sensor Bay
Chamber Heater(s) Location Sides, Bottom, Top and Back of Chamber
Chamber Jacket Technology Air Jacket via Two Axial Fans Drawing Air into Shell
CO2 Accuracy ± 0.1%
CO2 Control Logic Fixed Algorithm/Manual, Environmental Adaptable
CO2 Display Resolution 0.1%
CO2 Range 0.1 to 20%, 5% Default Set Point
CO2 Recovery Up to 5% ± 0.2% / -0.5 in 5 minutes Average
CO2 Sensor Type Infrared (NDIR)
Control System NuTouch
Data Output Features RS-485, USB, 4-20mA
Door and Perimeter Heater Control Logic Base Duty Cycle set to chamber temperature Set-Point, Proportional 0-100% [manually adjustable
Electrical Configuration 115V50Hz, 230V5060Hz
Exterior Depth Inches (mm) 27.188 (691)
Exterior Door Seal Magnetic Outer Door Gasket
Exterior Height Inches (mm) 39.688 (1008)
Exterior Material Cold Rolled 16 & 18 Gauge Steel, Finished in Powder Coated Polyurethane Finish
Exterior Width Inches (mm) 26.750 (680)
Footprint Depth inches (mm) 20.75 (527)
Footprint Width inches (mm) 23.75 (603)
In-line Gas Filters 99.97% Efficiency @ ≥ 0.3 Micron Disk Filters for Gas & Air Supplies, Disk Filter for CO2 Sensor
Segmented Doors Optional Set of 6 Inner Doors, 2 Across by 3 Down
Inner Doors One, Sectioned Optional
Insulation High Density Insulation with High "R" Rating
Interior Depth Inches (mm) 20.053 (509)
Interior Door Seal Silicone Rubber Gasket
Interior Height Inches (mm) 28.500 (724)
Interior Material Highly Polished Type 304L 16 Gauge Stainless Steel
Interior Volume Cubic Feet (Liters) 7.06 (200)
Interior Width Inches (mm) 21.257 (540)
Net Weight lbs (kg) 235 (106)
O2 Accuracy ± 0.25%
O2 Control Logic Fixed algorithm/ manual environmental adaptable
O2 Display Resolution 0.1%
O2 Measuring Range 0 - 25%
O2 Set Point Range 0.5 -21.0% (ambient) (21.0% Default)
O2 Recovery 5.0 ± 0.5% twenty minutes on Average
O2 Sensor Type Zirconia Ceramic
Power Cord Length Foot (Meter) 8 (2.5)
Remote Alarm Terminals Standard
RH Accuracy
RH Control Method
RH Control Logic
RH Control Range
RH Display Resolution
RH Evaporator Box Capacity
RH Recovery
RH Sensor Type
RH Water Pan Capacity
Sample Port Location Inner Glass Door
Shelf Capacity lbs (kg) 25 lbs per Shelf / 125 lbs per Incubator
Shelf Depth Inches (mm) 18 3/4 (476)
Shelf Material Stainless Steel or Copper Alloy
Shelf Width Inches (mm) 19 3/4 (502)
Shelves 4 Shelves Supplied, 20 Shelves Maximum Capacity
Est. Shipping Weight lbs (kg) 325 (147)
Decontamination Cycle 95°C Humidified Decontamination, 145°C Dry Decontamination Cycle
Temperature Accuracy °C ± 0.1%
Temperature Control Direct Heat
Temperature Control Range 5°C above ambient (to a 30°C max ambient) to 55°C
Temperature Display Resolution 0.1°C
Temperature Recovery 0.12°C/min. on Average
Temperature Sensor Type Precision Integrated Circuit
Temperature Set-Point Range 5°C to 55°C (37.0°C Default)
Temperature Uniformity °C ± 0.35°C @ 37°C
Water Pan Depth 1.5 (38)
Water Pan Length Inches (mm) 12 (305)
Water Pan Width 10 (254)


NU-5831 NU-5831E
Electrical Configuration 115V60Hz 230V5060Hz


NU-5831 NU-5831E
Safety Certification [ "", "UL, UL-C" ] [ "", "UL, UL-C" ]


Copper Alloy Chamber 115V
Copper Alloy Chamber 230V
Stainless Steel Chamber 115V
Stainless Steel Chamber 230V

Inner Door

Inner Glass Door
Sectioned Inner Door

Outer Door (Right Hinge Standard)

Left Side Door Hinge

Power Cord

Euro Power Cord Option Shipping Kit
UK Power Cord Option

NuAire Accessories

Please keep in mind parts and accessories are product and size specific, contact your sales representative for any clarity to your size questions.
Brochure In-VitroCell CO2 Incubator Brochure

In-VitroCell CO2 Incubator Brochure

Download the brochure
Warranty NuAire Standard CO2 Incubator Warranty in United States and Canada

CO2 Incubator Standard Warranty for US and CA

View the warranty
White Paper CO2 Incubator Buying Guide

CO2 Incubator Buying Guide

Learn More
Specification CO2 Incubator Direct Heat NU-5800 Family Specification

CO2 Incubator Direct Heat NU-5800 Family Specification

View the specification
Video In-VitroCell CO2 Incubators Temperature Uniformity

In-VitroCell CO2 Incubators Temperature Uniformity

Watch the video
Video In-VitroCell CO2 Incubator Closed Loop HEPA Filtration

In-VitroCell CO2 Incubator Closed Loop HEPA Filtration

Watch the video
Video CO2 Incubators NuTouch Electronic Control System

In-VitroCell CO2 Incubators NuTouch Electronic Control System

Watch the video
BIM Revit

NU-5800 Family of Direct Heat CO2 Incubators 3D Model

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BIM Revit

NU-5700 Family Stacked on NU-5800 Family of Direct Heat CO2 Incubator 3D Model

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Video In-VitroCell CO2 Incubators IR Sensor

In-VitroCell CO2 Incubators IR Sensor

Watch the video
Video In-VitroCell CO2 Incubators from NuAire Lab Equipment

In-VitroCell CO2 Incubators from NuAire

Watch the video
Video CO2 Incubator Contamination Control

CO2 Incubators Constant Contamination Control

Watch the video

NU-5700 stacked on NU-5800 CO2 Incubator

View the drawing

CO2 Incubator Cut Sheet for models NU-5800, NU-5810, NU-5820, NU-5831, NU-5841

View the drawing
Manuals Operation and maintenance manual for NU-5800 and NU-5800E Series 12 CO2 Incubator

OM0245 NU-5800 and NU-5800E Series 12 CO2 Incubator Manual

Read the manual
Manuals OM0245 CO2 Incubator Manual for models NU-5810, NU-5820, NU-5831, and NU-5841

OM0246 CO2 Incubator Manual for NU-5810/E through NU-5841/E

Read the manual



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